Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick.

ABSTRACT: BACKGROUND:MiR-9 is a small non-coding RNA that is highly conserved between species and primarily expressed in the central nervous system (CNS). It is known to influence proliferation and neuronal differentiation in the brain and spinal cord of different vertebrates. Different studies have pointed to regional and species-specific differences in the response of neural progenitors to miR-9. METHODS:In ovo and ex ovo electroporation was used to overexpress or reduce miR-9 followed by mRNA in situ hybridisation and immunofluorescent stainings to evaluate miR- expression and the effect of changed miR-9 expression. RESULTS:We have investigated the expression and function of miR-9 during early development of the mid-hindbrain region (MH) in chick. Our analysis reveals a closer relationship of chi

SUBMITTER: Alwin Prem Anand A 

PROVIDER: S-EPMC5824543 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature