Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction.

ABSTRACT:

Background

Brain pericytes ensheathe the endothelium and contribute to formation and maintenance of the blood-brain-barrier. Additionally, pericytes are involved in several aspects of the CNS immune response including scarring, adhesion molecule expression, chemokine secretion, and phagocytosis. In vitro cultures are routinely used to investigate these functions of brain pericytes, however, these are highly plastic cells and can display differing phenotypes and functional responses depending on their culture conditions. Here we sought to investigate how two commonly used culture media, high serum containing DMEM/F12 and low serum containing Pericyte Medium (ScienCell), altered the phenotype of human brain pericytes and neuroinflammatory responses.

Methods

Pericytes were isol

SUBMITTER: Rustenhoven J 

PROVIDER: S-EPMC5824614 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature