Comparative transcriptomics of multidrug-resistant Acinetobacter baumannii in response to antibiotic treatments.

ABSTRACT: Multidrug-resistant Acinetobacter baumannii, a major hospital-acquired pathogen, is a serious health threat and poses a great challenge to healthcare providers. Although there have been many genomic studies on the evolution and antibiotic resistance of this species, there have been very limited transcriptome studies on its responses to antibiotics. We conducted a comparative transcriptomic study on 12 strains with different growth rates and antibiotic resistance profiles, including 3 fast-growing pan-drug-resistant strains, under separate treatment with 3 antibiotics, namely amikacin, imipenem, and meropenem. We performed deep sequencing using a strand-specific RNA-sequencing protocol, and used de novo transcriptome assembly to analyze gene expression in the form of polycistronic transcrip

SUBMITTER: Qin H 

PROVIDER: S-EPMC5824817 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature