Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations.

ABSTRACT: Employing selenocysteine-containing protein fragments to form the amide bond between respective protein fragments significantly extends the current capabilities of the widely used protein engineering method, expressed protein ligation. Selenocysteine-mediated ligation is noteworthy for its high yield and efficiency. However, it has so far been restricted to solid-phase synthesized seleno-peptides and thus constrained by where the selenocysteine can be positioned. Here we employ heterologously expressed seleno-fragments to overcome the placement and size restrictions in selenocysteine-mediated chemical ligation. Following ligation, the selenocysteine can be deselenized into an alanine or serine, resulting in nonselenoproteins. This greatly extends the flexibility in selecting the conjugatio

SUBMITTER: Liu J 

PROVIDER: S-EPMC5824972 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature