Self-assembled liver organoids recapitulate hepatobiliary organogenesis in vitro.

ABSTRACT: Several three-dimensional cell culture systems are currently available to create liver organoids. In gneral, these systems display better physiologic and metabolic aspects of intact liver tissue compared with two-dimensional culture systems. However, none reliably mimic human liver development, including parallel formation of hepatocyte and cholangiocyte anatomical structures. Here, we show that human fetal liver progenitor cells self-assembled inside acellular liver extracellular matrix scaffolds to form three-dimensional liver organoids that recapitulated several aspects of hepatobiliary organogenesis and resulted in concomitant formation of progressively more differentiated hepatocytes and bile duct structures. The duct morphogenesis process was interrupted by inhibiting Notch signaling

SUBMITTER: Vyas D 

PROVIDER: S-EPMC5825235 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature