Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis.

ABSTRACT: Most biosensors relying on antibodies as recognition elements fail in harsh environment conditions such as elevated temperatures, organic solvents, or proteases because of antibody denaturation, and require strict storage conditions with defined shelf life, thus limiting their applications in point-of-care and resource-limited settings. Here, a metal-organic framework (MOF) encapsulation is utilized to preserve the biofunctionality of antibodies conjugated to nanotransducers. This study investigates several parameters of MOF coating (including growth time, surface morphology, thickness, and precursor concentrations) that determine the preservation efficacy against different protein denaturing conditions in both dry and wet environments. A plasmonic biosensor based on gold nanorods as the n

SUBMITTER: Wang C 

PROVIDER: S-EPMC5825292 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature