Involvement of CYP1B1 in interferon γ-induced alterations of epithelial barrier integrity.

ABSTRACT:

Background and purpose

CYP1B1 and CYP1A1 are important extra-hepatic cytochromes, expressed in the colon and involved in the metabolism of dietary constituents and exogenous compounds. CYP1B1 expression is increased by pro-inflammatory cytokines, and it has been recently implicated in regulation of blood brain barrier function. We investigated its involvement in the increased permeability of the intestinal epithelial barrier observed in inflammatory conditions.

Experimental approach

Epithelial monolayers formed by human T84 colon carcinoma cells cultured on transwells, were disrupted by incubation with IFNγ (10 ng·mL^-1 ). Monolayer integrity was measured using transepithelial electrical resistance. CYP1A1 and CYP1B1 inhibitors or inducers were applied apically. Po

SUBMITTER: Alhouayek M 

PROVIDER: S-EPMC5825299 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature