Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos.

ABSTRACT: For decades, in situ hybridization methods have been essential tools for studies of vertebrate development and disease, as they enable qualitative analyses of mRNA expression in an anatomical context. Quantitative mRNA analyses typically sacrifice the anatomy, relying on embryo microdissection, dissociation, cell sorting and/or homogenization. Here, we eliminate the trade-off between quantitation and anatomical context, using quantitative in situ hybridization chain reaction (qHCR) to perform accurate and precise relative quantitation of mRNA expression with subcellular resolution within whole-mount vertebrate embryos. Gene expression can be queried in two directions: read-out from anatomical space to expression space reveals co-expression relationships in selected regions of

SUBMITTER: Trivedi V 

PROVIDER: S-EPMC5825878 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature