Regulatory T-Cells Mediate IFN-α-Induced Resistance against Antigen-Induced Arthritis.

ABSTRACT:

Objective

CD4⁺FoxP3⁺CD25⁺ regulatory T-cells (T_regs) are important for preventing tissue destruction. Here, we investigate the role of T_regs for protection against experimental arthritis by IFN-α.

Methods

Arthritis was triggered by intra-articular injection of methylated bovine serum albumin (mBSA) in wild-type mice, Foxp3DTReGFP^+/- mice [allowing selective depletion of T_regs by diphtheria toxin (DT)] and CD4-Cre^+/- IFNA1R flox/flox mice (devoid of IFNAR signaling in T-cells) earlier immunized with mBSA, with or without treatment with IFN-α or the indoleamine 2,3-dioxygenase (IDO)-metabolite kynurenine. T_regs were depleted in DT-treated Foxp3DTReGFP^+/- mice and enume

SUBMITTER: Chenna Narendra S 

PROVIDER: S-EPMC5826073 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature