CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo.

ABSTRACT: During development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibility changes in the development of somatic lineages are not well defined. Here we show that cell-specific chromatin accessibility data can be produced via ectopic expression of E. coli Dam methylase in vivo, without the requirement for cell-sorting (CATaDa). We have profiled chromatin accessibility in individual cell-types of Drosophila neural and midgut lineages. Functional cell-type-specific enhancers were identified, as well as novel motifs enriched at different stages of development. Finally

SUBMITTER: Aughey GN 

PROVIDER: S-EPMC5826290 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature