Comparative and network-based proteomic analysis of low dose ethanol- and lipopolysaccharide-induced macrophages.

ABSTRACT: Macrophages are specialized phagocytes that play an essential role in inflammation, immunity, and tissue repair. Profiling the global proteomic response of macrophages to microbial molecules such as bacterial lipopolysaccharide is key to understanding fundamental mechanisms of inflammatory disease. Ethanol is a widely abused substance that has complex effects on inflammation. Reports have indicated that ethanol can activate or inhibit the lipopolysaccharide receptor, Toll-like Receptor 4, in different settings, with important consequences for liver and neurologic inflammation, but the underlying mechanisms are poorly understood. To profile the sequential effect of low dose ethanol and lipopolysaccharide on macrophages, a gel-free proteomic technique was applied to RAW 264.7 macrophages. Fi

SUBMITTER: Kamal AHM 

PROVIDER: S-EPMC5826526 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature