Engineered Tissue Folding by Mechanical Compaction of the Mesenchyme.

ABSTRACT: Many tissues fold into complex shapes during development. Controlling this process in vitro would represent an important advance for tissue engineering. We use embryonic tissue explants, finite element modeling, and 3D cell-patterning techniques to show that mechanical compaction of the extracellular matrix during mesenchymal condensation is sufficient to drive tissue folding along programmed trajectories. The process requires cell contractility, generates strains at tissue interfaces, and causes patterns of collagen alignment around and between condensates. Aligned collagen fibers support elevated tensions that promote the folding of interfaces along paths that can be predicted by modeling. We demonstrate the robustness and versatility of this strategy for sculpting tissue interfaces by d

SUBMITTER: Hughes AJ 

PROVIDER: S-EPMC5826757 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature