Functional Characterization of Human Peptide/Histidine Transporter 1 in Stably Transfected MDCK Cells.

ABSTRACT: The proton-coupled oligopeptide transporter PHT1 (SLC15A4), which facilitates cross-membrane transport of histidine and small peptides from inside the endosomes or lysosomes to cytosol, plays an important role in intracellular peptides homeostasis and innate immune responses. However, it remains a challenge to elucidate functional properties of the PHT1 transporter because of its subcellular localization. The purpose of this study was to resort hPHT1 protein from the subcellular to outer cell membrane of MDCK cells stably transfected with human PHT1 mutants, and to characterize its functional activity in these cells. Using this model, the functional activity of hPHT1 was evaluated by cellular uptake studies with d₃-l-histidine, GlySar, and the bacterial peptidoglycan products MD

SUBMITTER: Song F 

PROVIDER: S-EPMC5826766 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature