Polymerization of hexamethylene diisocyanate in solution and a 260.23 m/z [M+H]⁺ ion in exposed human cells.

ABSTRACT: Hexamethylene diisocyanate (HDI) is an important industrial chemical that can cause asthma, however pathogenic mechanisms remain unclear. Upon entry into the respiratory tract, HDI's N=C=O groups may undergo nucleophilic addition (conjugate) to host molecules (e.g. proteins), or instead react with water (hydrolyze), releasing CO₂ and leaving a primary amine in place of the original N=C=O. We hypothesized that (primary amine groups present on) hydrolyzed or partially hydrolyzed HDI may compete with proteins and water as a reaction target for HDI in solution, resulting in polymers that could be identified and characterized using LC-MS and LC-MS/MS. Analysis of the reaction products formed when HDI was mixed with a pH buffered, isotonic, protein containing solution identified multi

SUBMITTER: Wisnewski AV 

PROVIDER: S-EPMC5826792 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature