Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics.

ABSTRACT: Toward the elucidation of the advanced mechanism of L-valine production by Corynebacterium glutamicum, a highly developed industrial strain VWB-1 was analyzed, employing the combination of transcriptomics and proteomics methods. The transcriptional level of 1155 genes and expression abundance of 96 proteins were changed significantly by the transcriptome and proteome comparison of VWB-1 and ATCC 13869. It was indicated that the key genes involved in the biosynthesis of L-valine, ilvBN, ilvC, ilvD, ilvE were up-regulated in VWB-1, which together made prominent contributions in improving the carbon flow towards L-valine. The L-leucine and L-isoleucine synthesis ability were weakened according to the down-regulation of leuB and ilvA. The up-regulation of the branched chain amino acid transpor

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5827029 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature