Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery.

ABSTRACT: Secretory granules released by cytotoxic T lymphocytes (CTLs) are powerful weapons against intracellular microbes and tumor cells. Despite significant progress, there is still limited information on the molecular mechanisms implicated in target-driven degranulation, effector cell survival and composition and structure of the lytic granules. Here, using a proteomic approach we identified a panel of putative cytotoxic granule proteins, including some already known granule constituents and novel proteins that contribute to regulate the CTL lytic machinery. Particularly, we identified galectin-1 (Gal1), an endogenous immune regulatory lectin, as an integral component of the secretory granule machinery and unveil the unexpected function of this lectin in regulating CTL killing activity. Mechani

SUBMITTER: Clemente T 

PROVIDER: S-EPMC5827204 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature