A functional link between NAD⁺ homeostasis and N-terminal protein acetylation in Saccharomyces cerevisiae.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) is an essential metabolite participating in cellular redox chemistry and signaling, and the complex regulation of NAD⁺ metabolism is not yet fully understood. To investigate this, we established a NAD⁺-intermediate specific reporter system to identify factors required for salvage of metabolically linked nicotinamide (NAM) and nicotinic acid (NA). Mutants lacking components of the NatB complex, NAT3 and MDM20, appeared as hits in this screen. NatB is an N^α-terminal acetyltransferase responsible for acetylation of the N terminus of specific Met-retained peptides. In NatB mutants, increased NA/NAM levels were concomitant with decreased NAD⁺ We identified the vacuolar pool of nicotinam

SUBMITTER: Croft T 

PROVIDER: S-EPMC5827443 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature