Identification of <i>Euglena gracilis</i> β-1,3-glucan phosphorylase and establishment of a new glycoside hydrolase (GH) family GH149.

ABSTRACT: Glycoside phosphorylases (EC 2.4.x.x) carry out the reversible phosphorolysis of glucan polymers, producing the corresponding sugar 1-phosphate and a shortened glycan chain. β-1,3-Glucan phosphorylase activities have been reported in the photosynthetic euglenozoan Euglena gracilis, but the cognate protein sequences have not been identified to date. Continuing our efforts to understand the glycobiology of E. gracilis, we identified a candidate phosphorylase sequence, designated EgP1, by proteomic analysis of an enriched cellular protein lysate. We expressed recombinant EgP1 in Escherichia coli and characterized it in vitro as a β-1,3-glucan phosphorylase. BLASTP identified several hundred EgP1 orthologs, most of which were from Gram-negative bacteria and had 37-9

SUBMITTER: Kuhaudomlarp S 

PROVIDER: S-EPMC5827456 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature