Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement.

ABSTRACT: Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer's theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be de

SUBMITTER: Shi YZ 

PROVIDER: S-EPMC5827716 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature