RNA Polymerase Tags To Monitor Multidimensional Protein-Protein Interactions Reveal Pharmacological Engagement of Bcl-2 Proteins.

ABSTRACT: We report the development of a new technology for monitoring multidimensional protein-protein interactions (PPIs) inside live mammalian cells using split RNA polymerase (RNAP) tags. In this new system, a protein-of-interest is tagged with an N-terminal split RNAP (RNAP_N), and multiple potential binding partners are each fused to orthogonal C-terminal RNAPs (RNAP_C). Assembly of RNAP_N with each RNAP_C is highly dependent on interactions between the tagged proteins. Each PPI-mediated RNAP_N-RNAP_C assembly transcribes from a separate promoter on a supplied DNA substrate, thereby generating a unique RNA output signal for each PPI. We develop and validate this new approach in the context of the Bcl-2 family of proteins. These key re

SUBMITTER: Pu J 

PROVIDER: S-EPMC5828006 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature