Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.

ABSTRACT:

Background

Biomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors. However, their relative importance remains challenging to evaluate. Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples. A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.

Results

The pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3

SUBMITTER: Herbaut M 

PROVIDER: S-EPMC5828075 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature