Overexpression of p54^nrb/NONO induces differential EPHA6 splicing and contributes to castration-resistant prostate cancer growth.

ABSTRACT: The non-POU domain-containing octamer binding protein p54^nrb/NONO is a multifunctional nuclear protein involved in RNA splicing, processing, and transcriptional regulation of nuclear hormone receptors. Through chromosome copy number analysis via whole-exome sequencing, we revealed amplification of the chromosome Xq11.22-q21.33 locus containing the androgen receptor (AR) and NONO genes in androgen-independent, castration-resistant prostate cancer (CRPC)-like LNCaP-SF cells. Moreover, NONO was frequently amplified and overexpressed in patients with CRPC. RNA sequencing data revealed that a truncated ephrin type-A receptor 6 (EPHA6) splice variant (EPHA6-001) was overexpressed in LNCaP-SF cells, and knockdown of NONO or EPHA6-001 pre

SUBMITTER: Yamamoto R 

PROVIDER: S-EPMC5828187 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature