Upregulation of lactate-inducible snail protein suppresses oncogene-mediated senescence through p16^INK4a inactivation.

ABSTRACT:

Background

The preferential use of aerobic glycolysis by tumor cells lead to high accumulation of lactate in tumor microenvironment. Clinical evidence has linked elevated lactate concentration with cancer outcomes. However, the role and molecular mechanisms of lactate in cellular senescence and tumor progression remain elusive.

Methods

The function of Snail in lactate-induced EMT in lung cancer cells was explored by wound healing assay and cell invasion assay. The qRT-PCR and dual luciferase reporter assay were performed to investigate how lactate regulates Snail expression. The level of TGF-β1 in culture supernatant of cells was measured by ELISA for its correlation with extracellular levels of lactate. Ras activity assay and SA-β-gal activity assay were established to dete

SUBMITTER: Li X 

PROVIDER: S-EPMC5828408 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature