Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes.

ABSTRACT: Single-channel recordings are widely used to explore functional properties of ion channels. Typically, such recordings are performed at bandwidths of less than 10 kHz because of signal-to-noise considerations, limiting the temporal resolution available for studying fast gating dynamics to greater than 100 µs. Here we present experimental methods that directly integrate suspended lipid bilayers with high-bandwidth, low-noise transimpedance amplifiers based on complementary metal-oxide-semiconductor (CMOS) integrated circuits (IC) technology to achieve bandwidths in excess of 500 kHz and microsecond temporal resolution. We use this CMOS-integrated bilayer system to study the type 1 ryanodine receptor (RyR1), a Ca²⁺-activated intracellular Ca²⁺-release channel located on

SUBMITTER: Hartel AJW 

PROVIDER: S-EPMC5828579 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature