Accelerated wound healing in mice by on-site production and delivery of CXCL12 by transformed lactic acid bacteria.

ABSTRACT: Impaired wound closure is a growing medical problem associated with metabolic diseases and aging. Immune cells play important roles in wound healing by following instructions from the microenvironment. Here, we developed a technology to bioengineer the wound microenvironment and enhance healing abilities of the immune cells. This resulted in strongly accelerated wound healing and was achieved by transforming Lactobacilli with a plasmid encoding CXCL12. CXCL12-delivering bacteria administrated topically to wounds in mice efficiently enhanced wound closure by increasing proliferation of dermal cells and macrophages, and led to increased TGF-β expression in macrophages. Bacteria-produced lactic acid reduced the local pH, which inhibited the peptidase CD26 and consequently enhanced the availab

SUBMITTER: Vagesjo E 

PROVIDER: S-EPMC5828606 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature