Proteomic Analysis Shows Constitutive Secretion of MIF and p53-associated Activity of COX-2^-/- Lung Fibroblasts.

ABSTRACT: The differential expression of two closelyassociated cyclooxygenase isozymes, COX-1 and COX-2, exhibited functions beyond eicosanoid metabolism. We hypothesized that COX-1 or COX-2 knockout lung fibroblasts may display altered protein profiles which may allow us to further differentiate the functional roles of these isozymes at the molecular level. Proteomic analysis shows constitutive production of macrophage migration inhibitory factor (MIF) in lung fibroblasts derived from COX-2^-/- but not wild-type (WT) or COX-1^-/- mice. MIF was spontaneously released in high levels into the extracellular milieu of COX2^-/- fibroblasts seemingly from the preformed intracellular stores, with no change in the basal gene expression of MIF. The secretion and regulation of MI

SUBMITTER: Dave M 

PROVIDER: S-EPMC5828655 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature