Enhanced intracellular delivery via coordinated acoustically driven shear mechanoporation and electrophoretic insertion.

ABSTRACT: Delivery of large and structurally complex target molecules into cells is vital to the emerging areas of cellular modification and molecular therapy. Inadequacy of prevailing in vivo (viral) and in vitro (liposomal) gene transfer methods for delivery of proteins and a growing diversity of synthetic nanomaterials has encouraged development of alternative physical approaches. Efficacy of injury/diffusion-based delivery via shear mechanoporation is largely insensitive to cell type and target molecule; however, enhanced flexibility is typically accompanied by reduced gene transfer effectiveness. We detail a method to improve transfection efficiency through coordinated mechanical disruption of the cell membrane and electrophoretic insertion of DNA to the cell interior. An array of micromachined

SUBMITTER: Meacham JM 

PROVIDER: S-EPMC5829135 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature