A sigma factor toolbox for orthogonal gene expression in Escherichia coli.

ABSTRACT: Synthetic genetic sensors and circuits enable programmable control over timing and conditions of gene expression and, as a result, are increasingly incorporated into the control of complex and multi-gene pathways. Size and complexity of genetic circuits are growing, but stay limited by a shortage of regulatory parts that can be used without interference. Therefore, orthogonal expression and regulation systems are needed to minimize undesired crosstalk and allow for dynamic control of separate modules. This work presents a set of orthogonal expression systems for use in Escherichia coli based on heterologous sigma factors from Bacillus subtilis that recognize specific promoter sequences. Up to four of the analyzed sigma factors can be combined to function orthogonally between each other and

SUBMITTER: Bervoets I 

PROVIDER: S-EPMC5829568 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature