Myocardial Polyploidization Creates a Barrier to Heart Regeneration in Zebrafish.

ABSTRACT: Correlative evidence suggests that polyploidization of heart muscle, which occurs naturally in post-natal mammals, creates a barrier to heart regeneration. Here, we move beyond a correlation by demonstrating that experimental polyploidization of zebrafish cardiomyocytes is sufficient to suppress their proliferative potential during regeneration. Initially, we determined that zebrafish myocardium becomes susceptible to polyploidization upon transient cytokinesis inhibition mediated by dominant-negative Ect2. Using a transgenic strategy, we generated adult animals containing mosaic hearts composed of differentially labeled diploid and polyploid-enriched cardiomyocyte populations. Diploid cardiomyocytes outcompeted their polyploid neighbors in producing regenerated heart muscle. Moreover, hea

SUBMITTER: Gonzalez-Rosa JM 

PROVIDER: S-EPMC5830170 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature