A Transcriptional Sequencing Analysis of Islet Stellate Cell and Pancreatic Stellate Cell.

ABSTRACT:

Background

Our previous studies have shown that islet stellate cell (ISC), similar to pancreatic stellate cell (PSC) in phenotype and biological characters, may be responsible for the islet fibrosis in type 2 diabetes. To further identify the differences between PSC and ISC and for better understanding of the physiological function of ISC, we employed genome-wide transcriptional analysis on the PSCs and ISCs of Wistar rats.

Method

PSCs and ISCs from each rat were primarily cultured at the same condition. Genome-wide transcriptional sequence of stellate cells was generated. The identified differentially expressed genes were validated using RT-PCR.

Results

32 significant differentially expressed genes between PSCs and ISCs were identified. Moreover, collagen type 11a1 (

SUBMITTER: Wang X 

PROVIDER: S-EPMC5830286 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature