Development of an LC-MS/MS peptide mapping protocol for the NISTmAb.

ABSTRACT: Peptide mapping is a component of the analytical toolbox used within the biopharmaceutical industry to aid in the identity confirmation of a protein therapeutic and to monitor degradative events such as oxidation or deamidation. These methods offer the advantage of providing site-specific information regarding post-translational and chemical modifications that may arise during production, processing or storage. A number of such variations may also be induced by the sample preparation methods themselves which may confound the ability to accurately evaluate the true modification levels. One important focus when developing a peptide mapping method should therefore be the use of sample preparation conditions that will minimize the degree of artificial modifications induced. Unfortunately, the

SUBMITTER: Mouchahoir T 

PROVIDER: S-EPMC5830484 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature