LGR5 expression is regulated by EGF in early colorectal adenomas and governs EGFR inhibitor sensitivity.

ABSTRACT:

Background

LGR5 serves as a co-receptor for Wnt/β-catenin signalling and marks normal intestinal stem cells; however, its role in colorectal cancer (CRC) remains controversial. LGR5⁺ cells are known to exist outside the stem cell niche during CRC progression, and the requirement for epidermal growth factor (EGF) signalling within early adenomas remains to be fully elucidated.

Methods

Epidermal growth factor and gefitinib treatments were performed in EGF-responsive LGR5⁺ early adenoma RG/C2 cells. 2D growth assays were measured using an IncuCyte. LGR5 or MEK1/2 silencing studies were executed using siRNA and LGR5 expression was assessed by qRT-PCR and immunoblotting. Ki67 level and cell cycle status were analysed by flow cytometry.

Results

Epiderm

SUBMITTER: Morgan RG 

PROVIDER: S-EPMC5830587 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature