Protein kinase D1 regulates subcellular localisation and metastatic function of metastasis-associated protein 1.

ABSTRACT:

Background

Cancer progression and metastasis is profoundly influenced by protein kinase D1 (PKD1) and metastasis-associated protein 1 (MTA1) in addition to other pathways. However, the nature of regulatory relationship between the PKD1 and MTA1, and its resulting impact on cancer metastasis remains unknown. Here we present evidence to establish that PKD1 is an upstream regulatory kinase of MTA1.

Methods

Protein and mRNA expression of MTA1 in PKD1-overexpressing cells were determined using western blotting and reverse-transcription quantitative real-time PCR. Immunoprecipitation and proximity ligation assay (PLA) were used to determine the interaction between PKD1 and MTA1. PKD1-mediated nucleo-cytoplasmic export and polyubiquitin-dependent proteosomal degradation was determi

SUBMITTER: Ganju A 

PROVIDER: S-EPMC5830591 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature