A novel Epstein-Barr virus-latent membrane protein-1-specific T-cell receptor for TCR gene therapy.

ABSTRACT: BACKGROUND:Adoptive transfer of genetically engineered T-cells to express antigen-specific T-cell receptor (TCR) is a feasible and effective therapeutic approach for numerous types of cancers, including Epstein-Barr virus (EBV)-associated malignancies. Here, we describe a TCR gene transfer regimen to rapidly and reliably generate T-cells specific to EBV-encoded latent membrane protein-1 (LMP1), which is a potential target for T-cell-based immunotherapy. METHODS:A novel TCR specific to LMP1 (LMP1-TCR) was isolated from HLA-A*0201 transgenic mice that were immunised with the minimal epitope LMP1166 (TLLVDLLWL), and LMP1-TCR-transduced peripheral blood lymphocytes were evaluated for functional specificities. RESULTS:Both human CD8 and CD4 T-cells expressing the LMP1-TCR provoked high levels o

SUBMITTER: Cho HI 

PROVIDER: S-EPMC5830600 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature