Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells.

ABSTRACT: Various mesenchymal cell types have been identified as critical components of the hematopoietic stem/progenitor cell (HSPC) niche. Although several groups have described the generation of mesenchyme from human pluripotent stem cells (hPSCs), the capacity of such cells to support hematopoiesis has not been reported. Here, we demonstrate that distinct mesenchymal subpopulations co-emerge from mesoderm during hPSC differentiation. Despite co-expression of common mesenchymal markers (CD73, CD105, CD90, and PDGFRβ), a subset of cells defined as CD146^hiCD73^hi expressed genes associated with the HSPC niche and supported the maintenance of functional HSPCs ex vivo, while CD146^loCD73^lo cells supported differentiation. Stromal support of HSPCs was contact

SUBMITTER: Chin CJ 

PROVIDER: S-EPMC5830911 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature