Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B-cell programming.

ABSTRACT: B-cell fate determination requires the action of transcription factors that operate in a regulatory network to activate B-lineage genes and repress lineage-inappropriate genes. However, the dynamics and hierarchy of events in B-cell programming remain obscure. To uncouple the dynamics of transcription factor expression from functional consequences, we generated induction systems in developmentally arrested Ebf1^-/- pre-pro-B cells to allow precise experimental control of EBF1 expression in the genomic context of progenitor cells. Consistent with the described role of EBF1 as a pioneer transcription factor, we show in a time-resolved analysis that EBF1 occupancy coincides with EBF1 expression and precedes the formation of chromatin accessibility. We observed dynamic pattern

SUBMITTER: Li R 

PROVIDER: S-EPMC5830932 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature