Force determination in lateral magnetic tweezers combined with TIRF microscopy.

ABSTRACT: Combining single-molecule techniques with fluorescence microscopy has attracted much interest because it allows the correlation of mechanical measurements with directly visualized DNA : protein interactions. In particular, its combination with total internal reflection fluorescence microscopy (TIRF) is advantageous because of the high signal-to-noise ratio this technique achieves. This, however, requires stretching long DNA molecules across the surface of a flow cell to maximize polymer exposure to the excitation light. In this work, we develop a module to laterally stretch DNA molecules at a constant force, which can be easily implemented in regular or combined magnetic tweezers (MT)-TIRF setups. The pulling module is further characterized in standard flow cells of different thicknesses a

SUBMITTER: Madariaga-Marcos J 

PROVIDER: S-EPMC5831119 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature