Ligand-Mediated Receptor Assembly as an End Point for High-Throughput Chemical Toxicity Screening.

ABSTRACT: The high throughput screening of chemicals for interaction with intracellular targets is gaining prominence in the toxicity evaluation of environmental chemicals. We describe ligand-mediated receptor assembly as an early event in receptor signaling and its application to the screening of chemicals for interaction with targeted receptors. We utilized bioluminescence resonance energy transfer (BRET) to detect and quantify assembly of the methyl farnesoate receptor (MfR) in response to various high-production volume and other chemicals. The hormone methyl farnesoate binds to the MfR to regulate various aspects of reproduction and development in crustaceans. The MfR protein subunits Met and SRC, cloned from Daphnia pulex, were fused to the fluorophore, mAmetrine and the photon generator, Rluc2

SUBMITTER: Medlock Kakaley EK 

PROVIDER: S-EPMC5831241 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature