Harnessing macrophage-mediated degradation of gelatin microspheres for spatiotemporal control of BMP2 release.

ABSTRACT: Biomaterials-based approaches to harnessing the immune and inflammatory responses to potentiate wound healing hold important promise. Bone fracture healing is characterized by an acute inflammatory phase, followed by a transition to a regenerative and repair phase. In this study, we developed genipin-crosslinked gelatin microspheres designed to be preferentially degraded by inflammatory (M1) macrophages. Highly crosslinked (>90%) microspheres allowed efficient incorporation of bioactive bone morphogenetic protein 2 (BMP2), a potent stimulator of osteogenesis in progenitor cells, via electrostatic interactions. Release of BMP2 was directly correlated with degradation of the gelatin matrix. Exposure of microspheres to polarized murine macrophages showed that degradation was significantly hig

SUBMITTER: Annamalai RT 

PROVIDER: S-EPMC5831261 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature