ABCA1-Derived Nascent High-Density Lipoprotein-Apolipoprotein AI and Lipids Metabolically Segregate.

ABSTRACT:

Objective

Reverse cholesterol transport comprises cholesterol efflux from ABCA1-expressing macrophages to apolipoprotein (apo) AI, giving nascent high-density lipoprotein (nHDL), esterification of nHDL-free cholesterol (FC), selective hepatic extraction of HDL lipids, and hepatic conversion of HDL cholesterol to bile salts, which are excreted. We tested this model by identifying the fates of nHDL-[³H]FC, [¹⁴C] phospholipid (PL), and [¹²⁵I]apo AI in serum in vitro and in vivo.

Approach and results

During in vitro incubation of human serum, nHDL-[³H]FC and [¹⁴C]PL rapidly transfer to HDL and low-density lipoproteins (t_1/2=2-7 minutes), whereas nHDL-[¹²⁵I]apo AI transfers solely to HDL (t

SUBMITTER: Xu B 

PROVIDER: S-EPMC5831354 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature