Highly sensitive and selective determination of redox states of coenzymes Q₉ and Q₁₀ in mice tissues: Application of orbitrap mass spectrometry.

ABSTRACT: Coenzyme Q (CoQ) is a redox active molecule that plays a fundamental role in mitochondrial energy generation and functions as a potent endogenous antioxidant. Redox ratio of CoQ has been suggested as a good marker of mitochondrial dysfunction and oxidative stress. Nevertheless, simultaneous measurement of redox states of CoQ is challenging owing to its hydrophobicity and instability of the reduced form. In order to improve the analytical methodology, paying special attention to this instability, we developed a highly sensitive and selective high-resolution/accurate-mass (HR/AM) UHPLC-MS/MS method for the rapid determination of redox states of CoQ₉ and CoQ₁₀ by ultra-performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometry. CoQs were extracted usi

SUBMITTER: Pandey R 

PROVIDER: S-EPMC5831383 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature