Mn²⁺ modulates the expression of cellulase genes in Trichoderma reesei Rut-C30 via calcium signaling.

ABSTRACT:

Background

The filamentous fungus Trichoderma reesei Rut-C30 is one of the most vital fungi for the production of cellulases, which can be used for biofuel production from lignocellulose. Nevertheless, the mechanism of transmission of external stimuli and signals in modulating cellulase production in T. reesei Rut-C30 remains unclear. Calcium is a known second messenger regulating cellulase gene expression in T. reesei.

Results

In this study, we found that a biologically relevant extracellular Mn²⁺ concentration markedly stimulates cellulase production, total protein secretion, and the intracellular Mn²⁺ concentration of Rut-C30, a cellulase hyper-producing strain of T. reesei. Furthermore, we identified two Mn²⁺ t

SUBMITTER: Chen Y 

PROVIDER: S-EPMC5831609 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature