A novel canine histiocytic sarcoma cell line: initial characterization and utilization for drug screening studies.

ABSTRACT:

Background

Histiocytic sarcoma is a rare disorder in humans, however it is seen with appreciable frequency in certain breeds of dogs, such as Bernese mountain dog. The purpose of this study was to fully characterize a novel canine histiocytic sarcoma cell line, and utilize it as a tool to screen for potential therapeutic drugs.

Methods

The histiocytic sarcoma cell line was characterized by expression of cellular markers as determined by immunohistochemistry and flow cytometry techniques. The neoplastic cells were also evaluated for their capability of phagocytizing beads particles, and their potential to grow as xenograft in an immunodeficient mouse. We investigated the in vitro cytotoxic activity of a panel of thirteen compounds using the MTS proliferation assay. Inhibitory

SUBMITTER: Takada M 

PROVIDER: S-EPMC5831740 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature