Revealing the Amylase Interactome in Whole Saliva Using Proteomic Approaches.

ABSTRACT: Understanding proteins present in saliva and their function when isolated is not enough to describe their real role in the mouth. Due to protein-protein interactions, structural changes may occur in macromolecules leading to functional modulation or modification. Besides amylase's function in carbohydrate breakdown, amylase can delay proteolytic degradation of protein partners (e.g., histatin 1) when complexed. Due to its biochemical characteristics and high abundance in saliva, amylase probably interacts with several proteins acting as a biological carrier. This study focused on identifying interactions between amylase and other proteins found in whole saliva (WS) using proteomic approaches. Affinity chromatography was used, followed by gel electrophoresis methods, sodium dodecyl sulfate

SUBMITTER: Crosara KTB 

PROVIDER: S-EPMC5831883 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature