PO and ID BCG vaccination in humans induce distinct mucosal and systemic immune responses and CD4⁺ T cell transcriptomal molecular signatures.

ABSTRACT: Protective efficacy of Bacillus Calmette-Guérin (BCG) may be affected by the methods and routes of vaccine administration. We have studied the safety and immunogenicity of oral (PO) and/or intradermal (ID) administration of BCG in healthy human subjects. No major safety concerns were detected in the 68 healthy adults vaccinated with PO and/or ID BCG. Although both PO and ID BCG could induce systemic Th1 responses capable of IFN-γ production, ID BCG more strongly induced systemic Th1 responses. In contrast, stronger mucosal responses (TB-specific secretory IgA and bronchoalveolar lavage T cells) were induced by PO BCG vaccination. To generate preliminary data comparing the early gene signatures induced by mucosal and systemic BCG vaccination, CD4⁺ memory T cells were isolated fro

SUBMITTER: Hoft DF 

PROVIDER: S-EPMC5832504 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature