Large-scale patterning of single cells and cell clusters in hydrogels.

ABSTRACT: Biophysical properties of the extracellular matrix (ECM) are known to play a significant role in cell behavior. To gain a better understanding of the effects of the biophysical microenvironment on cell behavior, the practical challenge is longitudinally monitoring behavioral variations within a population to make statistically powerful assessments. Population-level measurements mask heterogeneity in cell responses, and large-scale individual cell measurements are often performed in a one-time, snapshot manner after removing cells from their matrix. Here we present an easy and low-cost method for large-scale, longitudinal studies of heterogeneous cell behavior in 3D hydrogel matrices. Using a platform we term "the drop-patterning chip", thousands of cells were simultaneously transferred fro

SUBMITTER: Gong X 

PROVIDER: S-EPMC5832855 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature