Altered eicosanoid production and phospholipid remodeling during cell culture.

ABSTRACT: The remodeling of PUFAs by the Lands cycle is responsible for the diversity of phospholipid molecular species found in cells. There have not been detailed studies of the alteration of phospholipid molecular species as a result of serum starvation or depletion of PUFAs that typically occurs during tissue culture. The time-dependent effect of cell culture on phospholipid molecular species in RAW 264.7 cells cultured for 24, 48, or 72 h was examined by lipidomic strategies. These cells were then stimulated to produce arachidonate metabolites derived from the cyclooxygenase pathway, thromboxane B₂, PGE₂, and PGD₂, and the 5-lipoxygenase pathway, leukotriene (LT)B₄, LTC₄, and 5-HETE, which decreased with increasing time in culture. However,

SUBMITTER: Okuno T 

PROVIDER: S-EPMC5832926 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature