PM2.5 promotes human bronchial smooth muscle cell migration via the sonic hedgehog signaling pathway.

ABSTRACT:

Background

The contribution of airway remodeling in chronic obstructive pulmonary disease (COPD) has been well documented, with airway smooth muscle cell proliferation and migration playing a role in the remodeling process. Here, we aimed to verify the effects of fine particulate matter (PM2.5) on human bronchial smooth muscle cell (HBSMC) migration and to explore the underlying signaling pathways.

Methods

HBSMC apoptosis, proliferation and migration were measured using flow cytometry, cell counting and transwell migration assays, respectively. The role of the hedgehog pathway in cell migration was assessed by western blotting to measure the expression of Sonic hedgehog (Shh), Gli1 and Snail. Furthermore, siRNA was used to knock down Gli1 or Snail expression.

Results

SUBMITTER: Ye X 

PROVIDER: S-EPMC5833105 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature