FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cells.

ABSTRACT: Rationale-endothelial cells (ECs) play important roles in various regeneration processes and can be used in a variety of therapeutic applications, such as cardiac regeneration, gene therapy, tissue-engineered vascular grafts and prevascularized tissue transplants. ECs can be acquired from pluripotent and adult stem cells. To acquire ECs from human embryonic stem cells (hESCs) in a fast, efficient and economic manner. We established a conditional overexpression system in hESCs based on 15 transcription factors reported to be responsible for hematopoiesis lineage. Among them, only overexpression of FLI1 could induce hESCs to a hematopoietic lineage. Moreover, simultaneous overexpression of FLI1 and activation of PKC rapidly and efficiently induced differentiation of hESCs into induced endoth

SUBMITTER: Zhao H 

PROVIDER: S-EPMC5833666 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature