Asymmetricity Between Sister Cells of Pluripotent Stem Cells at the Onset of Differentiation.

ABSTRACT: Various somatic stem cells divide asymmetrically; however, it is not known whether embryonic stem cells (ESCs) divide symmetrically or asymmetrically, not only while maintaining an undifferentiated state but also at the onset of differentiation. In this study, we observed single ESCs using time-lapse imaging and compared sister cell pairs derived from the same mother cell in either the maintenance or differentiation medium. Mouse ESCs were cultured on E-cadherin-coated glass-based dishes, which allowed us to trace single cells. The undifferentiated cell state was detected by green fluorescent protein (GFP) expression driven by the Nanog promoter, which is active only in undifferentiated cells. Cell population analysis using flow cytometry showed that the peak width indicating distribution

SUBMITTER: Nakamura S 

PROVIDER: S-EPMC5833898 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature